2020

Microbiome manipulation by a soil-borne fungal plant pathogen using effector proteins.
Snelders NC, Rovenich H, Petti GC, Rocafort M, van den Berg GCM, Vorholt JA, Mesters JR, Seidl MF, Nijland R, Thomma BPHJ.
Nat Plants. 2020 Nov;6(11):1365-1374. doi: 10.1038/s41477-020-00799-5. Epub 2020 Nov 2. PMID: 33139860

A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization.
Sánchez-Vallet A, Tian H, Rodriguez-Moreno L, Valkenburg DJ, Saleem-Batcha R, Wawra S, Kombrink A, Verhage L, de Jonge R, van Esse HP, Zuccaro A, Croll D, Mesters JR, Thomma BPHJ.
PLoS Pathog. 2020 Jun 23;16(6):e1008652. doi: 10.1371/journal.ppat.1008652. eCollection 2020 Jun. PMID: 32574207

Liver-expressed Cd302 and Cr1l limit hepatitis C virus cross-species transmission to mice.
Brown RJP, Tegtmeyer B, Sheldon J, Khera T, Anggakusuma, Todt D, Vieyres G, Weller R, Joecks S, Zhang Y, Sake S, Bankwitz D, Welsch K, Ginkel C, Engelmann M, Gerold G, Steinmann E, Yuan Q, Ott M, Vondran FWR, Krey T, Ströh LJ, Miskey C, Ivics Z, Herder V, Baumgärtner W, Lauber C, Seifert M, Tarr AW, McClure CP, Randall G, Baktash Y, Ploss A, Thi VLD, Michailidis E, Saeed M, Verhoye L, Meuleman P, Goedecke N, Wirth D, Rice CM, Pietschmann T.
Sci Adv. 2020 Nov 4;6(45):eabd3233. doi: 10.1126/sciadv.abd3233. Print 2020 Nov. PMID: 33148654

HCV Glycoprotein Structure and Implications for B-Cell Vaccine Development.
Ströh LJ, Krey T.
Int J Mol Sci. 2020 Sep 16;21(18):6781. doi: 10.3390/ijms21186781. PMID: 32947858

Global and local envelope protein dynamics of hepatitis C virus determine broad antibody sensitivity.
Augestad EH, Castelli M, Clementi N, Ströh LJ, Krey T, Burioni R, Mancini N, Bukh J, Prentoe J.
Sci Adv. 2020 Aug 26;6(35):eabb5938. doi: 10.1126/sciadv.abb5938. eCollection 2020 Aug. PMID: 32923643

CD81 extracted in SMALP nanodiscs comprises two distinct protein populations within a lipid environment enriched with negatively charged headgroups.
Ayub H, Clare M, Milic I, Chmel NP, Böning H, Devitt A, Krey T, Bill RM, Rothnie AJ.
Biochim Biophys Acta Biomembr. 2020 Nov 1;1862(11):183419. doi: 10.1016/j.bbamem.2020.183419. Epub 2020 Jul 28. PMID: 32735789

De novo protein design enables the precise induction of RSV-neutralizing antibodies.
Sesterhenn F, Yang C, Bonet J, Cramer JT, Wen X, Wang Y, Chiang CI, Abriata LA, Kucharska I, Castoro G, Vollers SS, Galloux M, Dheilly E, Rosset S, Corthésy P, Georgeon S, Villard M, Richard CA, Descamps D, Delgado T, Oricchio E, Rameix-Welti MA, Más V, Ervin S, Eléouët JF, Riffault S, Bates JT, Julien JP, Li Y, Jardetzky T, Krey T, Correia BE.
Science. 2020 May 15;368(6492):eaay5051. doi: 10.1126/science.aay5051. PMID: 32409444

Plant pathogen utilizes effector proteins to manipulate the host microbiome.
Snelders NC, Rovenich H, Petti GC, Rocafort Ferrer M, Mesters JR, Seidl MF, Nijland R, Thomma BPHJ.
Nature Plants. 2020 6, 1365–1374

Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors.
Zhang L, Lin D, Sun X, Curth U, Drosten C, Sauerhering L, Becker S, Rox K, Hilgenfeld R.
Science. 2020 368, 6489, 409-412. doi: 10.1126/science.abb3405

Processing of the SARS-CoV pp1a/ab nsp7-10 region.
Krichel B., Falke S, Hilgenfeld R, Redecke L, Uetrecht C.
Biochem J. 2020 477, 5 1009-1019. doi: 10.1042/BCJ20200029

Alpha-ketoamides as broad-spectrum inhibitors of coronavirus and enterovirus replication: Structure-based design, synthesis, and activity assessment.
Zhang L, Lin D, Kusov Y, Nian Y, Ma Q, Wang J, von Brunn A, Leyssen P, Lanko K, Neyts J, de Wilde A, Snijder EJ, Liu H, Hilgenfeld R.
J. Med. Chem.2020 63, 9 4562-4578. Biorxiv preprint server # 10.1101/2020.02.10.936898

Profiling of flaviviral NS2B-NS3 protease specificity provides a structural basis for the development of selective chemical tools that differentiate dengue from Zika and West Nile viruses.
Rut W, Groborz K, Zhang L, Modrzycka S, Poreba M, Hilgenfeld R, Drag M.
Antiviral Res. 2020 175, 104731. doi: 10.1016/j.antiviral.2020.104731

In cellulo crystallization of Trypanosoma brucei IMP dehydrogenase enables the identification of genuine co-factors.
Nass K, Redecke L, [...] Klinge M, [...] Schönherr R, Rehders D, Lahey-Rudolph JM, [...] Betzel C.
Nat Commun. 2020 11, 620

Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach.
Lahey-Rudolph JM, Schönherr R, Jeffries CM, Blanchet CE, Boger J, Ferreira Ramos AS, Riekehr WM, Triandafillidis D-P, Valmas A, Margiolaki I, Svergun D, Redecke L.
J. Appl. Cryst. 2020 53, 1169-1180, doi:10.1107/S1600576720010687