A new book on Drug Discovery

Publications 2019

A. Badolo, F.J. Burt, S. Daniel, R. Fearns, E.S. Gudo, M. Kielian, J. Lescar, Y. Shi, A. von Brunn, S.R. Weiss & R. Hilgenfeld:
Third Tofo Advanced Study Week on Emerging and Re-emerging Viruses.
Antiviral Res. 162, 142-150 (2019).

P. Havlickova, V. Brinsa, J. Brynda, P. Pachl, T. Prudnikova, J.R. Mesters, B. Kascakova, M. Kuty, M.L. Pusey, J.D. Ng, P. Rezacova & I. Kuta Smatanova:
A novel structurally characterized haloacid dehalogenase superfamily phosphatase from Thermococcus thioreducens with diverse substrate specificity.
Acta Cryst. D75, 743–752 (2019).

T. Prudnikova, B. Kascakova, J.R. Mesters, P. Grinkevich, P. Havlickova, A. Mazur, A. Shaposhnikova, R. Chaloupkova, J. Damborsky, M. Kuty & I. Kuta Smatanova:
Crystallization and Crystallographic Analysis of a Bradyrhizobium Elkanii USDA94 Haloalkane Dehalogenase Variant with an Eliminated Halide-Binding Site.
Crystals 9, 375 (2019).

Publications 2018

M.O. Wiedorn, ..., J.M. Lahey-Rudolph, ..., L. Redecke, ..., W.M. Riekehr, ..., R. Schönherr, ... & A. Barty:
Megahertz serial crystallography
Nature Communications 9, 4025 (2018).

R. Hilgenfeld & S.G. Vasudevan:
Dengue and Zika: Control and Antiviral Treatment Strategies.
Springer, ISBN 978-981-10-8727-1 (2018).

P. Grinkevich, D. Sinha, I. Iermak, A. Guzanova, M. Weiserova, J. Ludwig, J.R. Mesters & R. Ettrich:
Crystal structure of a novel domain of the motor subunit of the Type I restriction enzyme EcoR124 involved in complex assembly and DNA binding.
J. Biol. Chem. 293, 15043-15054 (2018).

R. Schönherr, J. M. Rudolph & L. Redecke:
Protein crystallization in living cells.
Biol. Chem. 399, 751–772 (2018).

R. Hilgenfeld, J. Lei & L. Zhang:
The Structure of the Zika Virus Protease, NS2B/NS3pro.
Adv. Exp. Med. Biol. 1062, 131-145 (2018).

A.M. de Silva, F.A. Rey, P.R. Young, R. Hilgenfeld & S.G. Vasudevan:
Viral Entry and NS1 as Potential Antiviral Drug Targets.
Adv. Exp. Med. Biol. 1062, 107-113 (2018).

J. Lei, Y. Kusov & R. Hilgenfeld:
Nsp3 of coronaviruses: Structures and functions of a large multi-domain protein.
Antiviral Res. 149, 58-74 (2018).

Publications 2017

H. Wang, M. Han, J. Qi, R. Hilgenfeld, T. Luo, Y. Shi, G.F. Gao & H. Song:
Crystal structure of the C-terminal fragment of NS1 protein from yellow fever virus.
Sci. China Life Sci. 60, 1403-1406 (2017).

K. Xiang, Y. Kusov, G. Ying, Y. Wang, Y. Shan, J. Wu, N. Yin, Y. Zhou, H. Li & M. Sun:
A Recombinant HAV Expressing a Neutralization Epitope of HEV Induces Immune Response against HAV and HEV in Mice.
Viruses 9, 260 (2017).

T. Schöne, L.L. Grimm, N. Sakai, L. Zhang, R. Hilgenfeld & T. Peters:
STD-NMR experiments identify a structural motif with novel second-site activity against West Nile virus NS2B-NS3 protease.
Antiviral Res. 146,174-183 (2017).

J. Lei & R. Hilgenfeld:
RNA-virus proteases counteracting host innate immunity.
FEBS Lett. 591, 3190-3210 (2017).

W.L. Tan, K.H. Wong, J. Lei, N. Sakai, H.W. Tan, R. Hilgenfeld & J.P. Tam:
Lybatides from Lycium barbarum contain an unusual cystine-stapled helical peptide scaffold.
Sci. Rep. 7, 5194 (2017).

J. Kouretova, M.Z. Hammamy, A. Epp, K. Hardes, S. Kallis, L. Zhang, R. Hilgenfeld, R. Bartenschlager & T. Steinmetzer:
Effects of NS2B-NS3 protease and furin inhibition on West Nile and Dengue virus replication.
J. Enzyme Inhib. Med. Chem. 32, 712-721 (2017).

S.S. Jadav, B.N. Sinha, R. Hilgenfeld & V. Jayaprakash:
Computer-Aided Structure Based Drug Design Approaches for the Discovery of New Anti-CHIKV Agents.
Curr. Comput. Aided Drug Des. 13, 346-361 (2017).

W. Rut, L. Zhang, P. Kasperkiewicz, M. Poreba, R. Hilgenfeld & M. Drąg:
Extended substrate specificity and first potent irreversible inhibitor/activity-based probe design for Zika virus NS2B-NS3 protease.
Antiviral Res. 139, 88-94 (2017).

C. Nitsche, L. Zhang, L.F. Weigel, J. Schilz, D. Graf, R. Bartenschlager, R. Hilgenfeld & C.D. Klein:
Peptide-Boronic Acid Inhibitors of Flaviviral Proteases: Medicinal Chemistry and Structural Biology.
J. Med. Chem. 60, 511-516 (2017).

Publications 2016

S. Fili, A. Valmas, M. Christopoulou, M. Spiliopoulou, N. Nikolopoulos, J. Lichière, S. Logotheti, F. Karavassili, E. Rosmaraki, A. Fitch, J. Wright, D. Beckers, T. Degen, G. Nénert, R. Hilgenfeld, N. Papageorgiou, B. Canard, B. Coutard & I. Margiolaki:
Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights.
Acta Cryst. F72, 877-884 (2016).

R. Hilgenfeld:
Zika virus NS1, a pathogenicity factor with many faces.
EMBO J. 35, 2631-2633 (2016).

P. Grinkevich, I. Iermak, N.A. Luedtke, J. R. Mesters, R. Ettrich & J. Ludwig:
pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I.
Acta Cryst. F Struct. Biol. Commun. 72, 672-676 (2016).

D. Becker, Z. Kaczmarska, C. Arkona, R. Schulz, C. Tauber, G. Wolber, R. Hilgenfeld, M. Coll & J. Rademann:
Irreversible inhibitors of the 3C protease of Coxsackie virus through templated assembly of protein-binding fragments.
Nat. Commun. 7,12761 (2016).

Y. Ma-Lauer, J. Carbajo-Lozoya, M.Y. Hein, M.A. Müller, W. Deng, J. Lei, B. Meyer, Y. Kusov, B. von Brunn, D.R. Bairad, S. Hünten, C. Drosten C, H. Hermeking, H. Leonhardt, M. Mann, R. Hilgenfeld & A. von Brunn:
p53 down-regulates SARS coronavirus replication and is targeted by the SARS-unique domain and PLpro via E3 ubiquitin ligase RCHY1.
Proc. Natl. Acad. Sci. USA, 113, 5192-5201 (2016).

J. Lei, G. Hansen, C. Nitsche, C. Klein, L. Zhang & R. Hilgenfeld:
Crystal structure of Zika virus NS2B-NS3 protease in complex with a boronate inhibitor.
Science, 353, 503-505 (2016).

J. Lei & R. Hilgenfeld:
Structural and mutational analysis of the interaction between the Middle-East respiratory syndrome coronavirus (MERS-CoV) papain-like protease and human ubiquitin.
Virol. Sin., 4, 288-299 (2016).

R. García-Fernández, P. Ziegelmüller, L. González, M. Mansur, Y. Machado, L. Redecke, U. Hahn, C. Betzel & M.L. Chávez:
Two variants of the major serine protease inhibitor from the sea anemone Stichodactyla helianthus, expressed in Pichia pastoris.
Protein Expr. Purif. 123, 42-50 (2016).

Z. Qin, Y. Xiao, X. Yang, J.R. Mesters, S. Yang & Z. Jiang:
A unique GCN5-related glucosamine N-acetyltransferase region exist in the fungal multi-domain glycoside hydrolase family
3 β-N-acetylglucosaminidase.
Sci. Rep. 5, 18292 (2016).

Publications 2015

K. Esser-Nobis, C. Harak, P. Schult, Y. Kusov & V. Lohmann:
Novel perspectives for hepatitis A virus therapy revealed by comparative analysis of hepatitis C virus and hepatitis A virus RNA replication.
Hepatology 62, 397-408 (2015).

F. Kohlmann, K. Shima, J. Rupp, W. Solbach, R. Hilgenfeld & G. Hansen:
Production, crystallization and X-ray diffraction analysis of the protease CT441 from Chlamydia trachomatis.
Acta Cryst. F71, 1454-1458 (2015).

L. Sun, A. Meijer, M. Froeyen, L. Zhang, H.J. Thibaut, J. Baggen, S. George, J. Vernachio, F.J. van Kuppeveld, P. Leyssen, R. Hilgenfeld, J. Neyts & L. Delang:
Antiviral activity of broad-spectrum and enterovirus-specific inhibitors against clinical isolates of enterovirus D68.
Antimicrob. Agents Chemother. 59, 7782-7785 (2015).

A. Schubert, R. Wrase, R. Hilgenfeld & G. Hansen:
Structures of DegQ from Legionella pneumophila define distinct ON and OFF states.
J. Mol. Biol. 427, 2840-2851 (2015).

M. Duszenko, L. Redecke, C.N. Mudogo, B.P. Sommer, S. Mogk, D. Oberthuer & C. Betzel:
In vivo protein crystallization in combination with highly brilliant radiation sources offers novel opportunities for the structural analysis of post-translationally modified eukaryotic proteins.
Acta Cryst. F71, 929-937 (2015).

H. Einspahr, I.K. Smatanova, C. Betzel & J.R. Mesters:
Introduction to selected articles from the 15th ICCBM.
Acta Cryst. F71, 805 (2015).

Y. Kusov, J. Tan, E. Alvarez, L. Enjuanes & R. Hilgenfeld:
A G-quadruplex-binding macrodomain within the "SARS-unique domain" is essential for the activity of the SARS-coronavirus replication-transcription complex.
Virology 484, 313-322 (2015).

C. Lacroix, S. George, P. Leyssen, R. Hilgenfeld & J. Neyts:
The enterovirus 3C protease inhibitor SG85 efficiently blocks rhinovirus replication and is not cross-resistant with rupintrivir.
Antimicrob. Agents Chemother. 59, 5814-5818 (2015).

R. Schönherr, M. Klinge, J. M. Rudolph, K. Fita, D. Rehders, F. Lübber, S. Schneegans, I. V. Majoul, M. Duszenko, C. Betzel, A. Brandariz-Nuñez, J. Martinez-Costas, R. Duden & L. Redecke:
Real-time investigation of dynamic protein crystallization in living cells.
Struct. Dyn. 2, 041712 (2015).

L. Galli, S.-K. Son, M. Klinge, S. Bajt, A. Barty, R. Bean, C. Betzel, K. R. Beyerlein, C. Caleman, R. B. Doak, M. Duszenko, H. Fleckenstein, C. Gati, B. Hunt, R. A. Kirian, M. Liang, M. H. Nanao, K. Nass, D. Oberthür, L. Redecke, R. Shoeman, F. Stellato, C. H. Yoon, T. A. White, O. Yefanov, J. Spence & H. N. Chapman:
Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse.
Struct. Dyn. 2, 041703 (2015).

R. García-Fernández, M. Perbandt, D. Rehders, P. Ziegelmüller, N. Piganeau, U. Hahn, C. Betzel, M. de L. Chávez & L. Redecke: Three-dimensional Structure of a Kunitz-type Inhibitor in Complex with an Elastase-like Enzyme.
J. Biol. Chem. 290,14154-14165 (2015).

Z. Han, N. Sakai, L.H. Böttger, S. Klinke, J. Hauber, A.X. Trautwein & R. Hilgenfeld:
Crystal Structure of the Peroxo-diiron(III) Intermediate of Deoxyhypusine Hydroxylase, an Oxygenase Involved in Hypusination.
Structure 23, 882-892 (2015).

O. Isken, U. Langerwisch, V. Jirasko, D. Rehders, L. Redecke, H. Ramanathan, B.D. Lindenbach, R. Bartenschlager & N. Tautz:
A Conserved NS3 Surface Patch Orchestrates NS2 Protease Stimulation, NS5A Hyperphosphorylation and HCV Genome Replication.
PLoS Pathog. 11, e1004736 (2015).

S.S. Jadav, B.N. Sinha, R. Hilgenfeld, B. Pastorino, X. de Lamballerie & V. Jayaprakash:
Thiazolidone derivatives as inhibitors of chikungunya virus.
Eur. J. Med. Chem. 89,172-178 (2015).

F. Kohlmann, K. Shima, R. Hilgenfeld, W. Solbach, J. Rupp & G. Hansen:
Structural basis of the proteolytic and chaperone activity of Chlamydia trachomatis CT441.
J. Bacteriol. 197, 211-218 (2015).

A. Sanchez-Vallet, J.R. Mesters & B.P.H.J. Thomma:
The battle for chitin recognition in plant-microbe interactions.
FEMS Microbiology Reviews. 39, 171-183 (2015).

A. Shukla & R. Hilgenfeld:
Acquisition of new protein domains by coronaviruses: analysis of overlapping genes coding for proteins N and 9b in SARS coronavirus.
Virus Genes 50, 29-38 (2015).

Publications 2014

K. Tepper, J. Biernat, S. Kumar, S. Wegmann, T. Timm, S. Hübschmann, L. Redecke, E.M. Mandelkow, D.J. Müller & E. Mandelkow:
Oligomer formation of tau protein hyperphosphorylated in cells.
J. Biol. Chem. 289, 34389-34407 (2014).

J.H. Hehemann , A. Law, L. Redecke & A.B. Boraston:
The structure of RdDddP from Roseobacter denitrificans reveals that DMSP lyases in the DddP-family are metalloenzymes.
PLoS One 9, e103128 (2014).

A. Tijsma, D. Franco, S. Tucker, R. Hilgenfeld, M. Froeyen, P. Leyssen & J. Neyts:
The capsid binder vapendavir and novel protease inhibitor SG85 inhibit enterovirus 71 replication.
Antimicrob. Agents Chemother. 58, 6990-6992 (2014).

R. Hilgenfeld:
From SARS to MERS: Crystallographic studies on coronaviral proteases enable antiviral drug design.
FEBS J. 281, 4085-4096 (2014).

D.X. Liu, T.S. Fung, K.K. Chong, A. Shukla & R. Hilgenfeld:
Accessory proteins of SARS-CoV and other coronaviruses.
Antiviral Res. 109, 97-109 (2014).

J. Lei, J.R. Mesters, C. Drosten, S. Anemüller, Q. Ma & R. Hilgenfeld:
Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features.
Antiviral Res. 109, 72-82 (2014).

J. Rocha-Pereira, M.S. Nascimento, Q. Ma, R. Hilgenfeld, J. Neyts & D. Jochmans:
The enterovirus protease inhibitor rupintrivir exerts broad-spectrum anti-norovirus activity and clears cells from the norovirus replicon.
Antimicrob. Agents Chemother. 58, 4675-4681 (2014).

R. Hilgenfeld, G. Seipke, H. Berchtold & D.R. Owens:
The Evolution of Insulin Glargine and its Continuing Contribution to Diabetes Care.
Drugs 74, 911-927 (2014).

M. Lahoda, J.R. Mesters, A. Stsiapanava, R. Chaloupkova, M. Kuty, J. Damborsky & I.K. Smatanova:
Crystallographic analysis of 1,2,3-trichloropropane biodegradation by the haloalkane dehalogenase DhaA31.
Acta Cryst. D70, 209-217 (2014).

C. Gati, G. Bourenkov, M. Klinge, D. Rehders, F. Stellato, D. Oberthür, O. Yefanov, B.P. Sommer, S. Mogk, M. Duszenko, C. Betzel, T.R. Schneider, H.N. Chapman & L. Redecke:
Serial crystallography on in vivo grown microcrystals using synchrotron radiation.
IUCr Journal 1, 87-94 (2014) - press release.

L. van der Linden, R. Ulferts, S.B. Nabuurs, Y. Kusov, H. Liu, S. George, C. Lacroix, N. Goris, D. Lefebvre, K.H. Lanke, K. De Clercq, R. Hilgenfeld, J. Neyts & F.J. van Kuppeveld:
Application of a cell-based protease assay for testing inhibitors of picornavirus 3C proteases.
Antiviral Res. 103, 17-24 (2014).

Publications 2013

L.C. Johansson et al. & L. Redecke et al. & R. Neutze:
Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography.
Nat. Commun. 4, 2911 (2013).

R. Hilgenfeld & M. Peiris:
From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses.
Antiviral Res. 100, 286–295 (2013).

M.I. Elmallah, U. Borgmeyer, C. Betzel & L. Redecke:
Impact of methionine oxidation as an initial event on the pathway of human prion protein conversion.
Prion 5, 404-411 (2013).

M. Uervirojnangkoorn, R. Hilgenfeld, T. Terwilliger & R. Read:
Improving experimental phases for strong reflections prior to density modification.
Acta Cryst. D69, 2039-2049 (2013).

P. Zhou, Z. Chen, Q. Yan, S. Yang, R. Hilgenfeld & Z. Jiang:
The structure of a glycoside hydrolase family 81 endo-β-1,3-glucanase.
Acta Cryst. D69, 2027-2038 (2013).

A. Sánchez-Vallet, R. Saleem-Batcha, A. Kombrink, G. Hansen, D.-J. Valkenburg, B.P.H.J. Thomma & J.R. Mesters:
Fungal effector Ecp6 outcompetes host immune receptor for chitin binding through intrachain LysM dimerization.
eLife 2, e00790 (2013).

F. Ye, J. Zhang, H. Liu, R. Hilgenfeld, R. Zhang, X. Kong, L. Li, J. Lu, X. Zhang, D. Li, H. Jiang, C.-G. Yang & C. Luo:
Helix unfolding/refolding characterizes the functional dynamics of Staphylococcus aureus Clp protease.
J. Biol. Chem. 288, 17643-17653 (2013).

J. Tan, S. George, Y. Kusov, M. Perbandt, S. Anemüller, J.R. Mesters, H. Norder, B. Coutard, C. Lacroix, P. Leyssen, J. Neyts & R. Hilgenfeld:
3C protease of enterovirus 68: Structure-based design of Michael acceptor inhibitors and their broad-spectrum antiviral effects against picornaviruses.
J. Virol. 87, 4339-4351 (2013).

L. Redecke et al.:
Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser.
Science 339, 227-230 (2013).

M.Z. Hammamy, C. Haase, M. Hammami, R. Hilgenfeld & T. Steinmetzer:
Development and characterization of new peptidomimetic inhibitors of the West Nile Virus NS2B-NS3 protease.
ChemMedChem. 8, 231-241 (2013).

G. Hansen & R. Hilgenfeld:
Architecture and regulation of HtrA-family proteins involved in protein quality control and stress response.
Cell. Mol. Life Sci. 70, 761-775 (2013).

Publications 2012

N. Redondo, M.A. Sanz, J. Steinberger, T. Skern, Y. Kusov & L. Carrasco:
Translation directed by Hepatitis A Virus in the absence of active eIF4F and eIF2.
PLoS ONE 12, e52065 (2012). doi:10.1371/journal.pone.0052065.

R. García-Fernández, T. Pons, M. Perbandt, P. A. Valiente, A. Talavera, Y. González-González, D. Rehders, M. A. Chávez,
C. Betzel & L. Redecke:
Structural insights into serine protease inhibition by a marine invertebrate BPTI Kunitz-type inhibitor.
J. Struct. Biol. 180, 271–279 (2012).

R. García-Fernández, T. Pons, A. Meyer, M. Perbandt, Y. González-González, D. Gil, M. A. Chávez, C. Betzel & L. Redecke:
Structure of the recombinant BPTI/Kunitz-type inhibitor rShPI-1A from the marine invertebrate Stichodactyla helianthus
Acta Cryst. F68, 1289–1293 (2012).

P.S. Lee, R. Yoshida, D.C. Ekiert, N. Sakai, Y. Suzuki, A. Takada & I.A. Wilson:
Heterosubtypic antibody recognition of the influenza virus hemagglutinin receptor binding site enhanced by avidity.
Proc. Natl. Acad. Sci. USA 42, 17040-17045 (2012).

Y. Ma-Lauer, J. Lei, R. Hilgenfeld & A. von Brunn:
Virus-host interactomes - antiviral drug discovery.
Curr. Opin. Virol. 2, 614-621 (2012).

B. Brix, J.R. Mesters, L. Pellerin & O. Jöhren:
Endothelial Cell-Derived Nitric Oxide Enhances Aerobic Glycolysis in Astrocytes via HIF-1α-Mediated Target Gene Activation.
J. Neurosci. 32, 9727-9735 (2012).

A. Meyer, K. Dierks, D. Hilterhaus, T. Klupsch, P. Mühlig, J. Kleesiek, R. Schöpflin, H. Einspahr, R. Hilgenfeld & C. Betzel:
Single-drop optimization of protein crystallization.
Acta Cryst. F68, 994-998 (2012).

E. Feng, D. Ye, J. Li, D. Zhang, J. Wang, F. Zhao, R. Hilgenfeld, M. Zheng, H. Jiang & H. Liu:
Recent Advances in Neuraminidase Inhibitor Development as Anti-influenza Drugs.
ChemMedChem. 7,1527-1536 (2012).

D.Z. Lin, W.K. Qian, R. Hilgenfeld, H.L. Jiang & H. Liu:
Improved synthesis of rupintrivir.
Science China Chem. 55, 1101-1107 (2012).

S. Boutet, ..., L. Redecke, ... & I. Schlichting:
High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography.
Science 337, 362-364 (2012).

G. Hansen & R. Hilgenfeld:
Recent structures of virulence proteins of Legionella establish targets for new antibacterials.
J. Microbes Infect. 7, 2-12 (2012).

H. Schmidt, G. Hansen, S. Singh, A. Hanuszkiewicz, B. Lindner, K. Fukase, R.W. Woodard, O. Holst, R. Hilgenfeld, U. Mamat & J.R. Mesters:
Structural and mechanistic analysis of the membrane-embedded glycosyltransferase WaaA required for lipopolysaccharide synthesis.
Proc. Natl. Acad. Sci. USA 109, 6253-6258 (2012).

Y. Xiao, Q. Ma, T. Restle, W. Shang, D.I. Svergun, R. Ponnusamy, G. Sczakiel & R. Hilgenfeld:
Non-structural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer that Exhibits Primer-independent RNA Polymerase Activity.
J. Virol. 86, 4444-4454 (2012).

R. Koopmann, K. Cupelli, L. Redecke et al. & D. Rehders et al....:
In vivo protein crystallization opens new routes in structural biology.
Nature Methods 9, 259-262 (2012).

Publications 2011

S. Pfefferle, J. Schöpf, M. Kögl, C. C. Friedel, M. A. Müller, J. Carbajo-Lozoya, T. Stellberger, E. von Dall’Armi, P. Herzog, S. Kallies, D. Niemeyer, V. Ditt, T. Kuri, R. Züst, K. Pumpor, R. Hilgenfeld, F. Schwarz, R. Zimmer, I. Steffen, F. Weber, V. Thiel, G. Herrler, H.-J. Thiel, C. Schwegmann-Weßels, S. Pöhlmann, J. Haas, C. Drosten & A. von Brunn:
The SARS-coronavirus-host interactome: Identification of cyclophilins as target for pan-coronavirus inhibitors
PLoS Pathog. 7, e1002331 (2011).

L. Zhu, S. George, M.F. Schmidt, S.I. Al-Gharabli, J. Rademann & R. Hilgenfeld:
Peptide aldehyde inhibitors challenge the substrate specificity of the SARS-coronavirus main protease.
Antiviral Res. 92, 204-212 (2011).

G. Hansen, B. Schwarzloh, A. Rennenberg, V. Heussler & R. Hilgenfeld:
The macromolecular complex of ICP and falcipain-2 from Plasmodium: preparation, crystallization, and preliminary X-ray diffraction analysis.
Acta Cryst. F67, 1406-1410 (2011).

S. Chen, Y. Xiao, R. Ponnusamy, J. Tan, J. Lei & R. Hilgenfeld:
X-ray structure of the SH3 domain of the phosphoinositide 3-kinase p85β subunit.
Acta Cryst. F67, 1328-1333 (2011).

H. Schmidt, J.R. Mesters, J. Wu, R.W. Woodard, R. Hilgenfeld & U. Mamat:
Evidence for a Two-Metal-Ion Mechanism in the Cytidyltransferase KdsB, an Enzyme Involved in Lipopolysaccharide Biosynthesis.
PLoS One. 6, e23231 (2011).

J. Zhang, A.M. Mamlouk, T. Martinetz, S. Chang, J. Wang & R. Hilgenfeld:
PhyloMap: an algorithm for visualizing relationships of large sequence data sets and its application to the influenza A virus genome.
BMC Bioinformatics 12, 248 (2011).

G. Hansen, A. Heitmann, T. Witt, H. Li, H. Jiang, X. Shen, V.T. Heussler, A. Rennenberg & R. Hilgenfeld:
Structural Basis for the Regulation of Cysteine-Protease Activity by a New Class of Protease Inhibitors in Plasmodium.
Structure 19, 919-929 (2011).

R. Wrase, H. Scott, R. Hilgenfeld & G. Hansen:
The Legionella HtrA homologue DegQ is a self-compartmentizing protease that forms large 12-meric assemblies.
Proc. Natl. Acad. Sci. USA 108, 10490-10495 (2011).

J. Lei, J.R. Mesters, A. von Brunn & R. Hilgenfeld:
Crystal structure of the middle domain of human poly(A)-binding protein-interacting protein 1.
Biochem. Biophys. Res. Commun. 408, 680-685 (2011).

A. Garcia-Caballero, J. Gavira, E. Pineda-Molina, N. Chayen, L. Govada, S. Kurshid, E. Saridakis, A. Boudjemline, M. Swann, P. Shaw Stewart, R. Briggs, S. Kolek, D. Oberthur, K. Dierks, C. Betzel, M. Santana, J. Hobbs, P. Thaw, T. Savill, J.R. Mesters, R. Hilgenfeld, N. Bonander & R. Bill:
Optimization of Protein Crystallization: The OptiCryst Project.
Cryst. Growth. Des. 11, 2112-2121 (2011).

J.R. Mesters & R. Hilgenfeld:
Glutamate Carboxypeptidase II
In: Handbook of Metalloproteins, Volumes 4 & 5, edited by Albrecht Messerschmidt. Chichester, UK: John Wiley & Sons, Ltd, pp 681-692 (2011).

R.N. Charrel, B. Coutard, C. Baronti, B. Canard, A. Nougairede, A. Frangeul, B. Morin, S. Jamal, C.L. Schmidt, R. Hilgenfeld, B. Klempa & X. de Lamballerie:
Arenaviruses and hantaviruses: From epidemiology and genomics to antivirals.
Antiviral Res. 90, 102-114 (2011).

H. Norder, A.M. De Palma, B. Selisko, L. Costenaro, N. Papageorgiou, C. Arnan, B. Coutard, V. Lantez, X. De Lamballerie,
C. Baronti, M. Solà, J. Tan, J. Neyts, B. Canard, M. Coll, A.E. Gorbalenya & R. Hilgenfeld:
Picornavirus non-structural proteins as targets for new anti-virals with broad activity.
Antiviral Res., 89, 204-218 (2011).

I. Lorenzen, W. Shang, M. Perbandt, M.V. Petoukhov, D.I. Svergun, G.H. Waetzig, S. Rose-John, R. Hilgenfeld & J. Grötzinger:
The structure of the unliganded extracellular domain of the interleukin-6 signal transducer gp130 in solution.
Eur. J. Cell Biol. 90, 515-520 (2011).

Publications 2010

R. Hilgenfeld:
Structure-based antivirals for emerging and neglected RNA viruses: an emerging field for medicinal chemistry in academia.
Future Med. Chem. 2, 1061-1067 (2010) (commentary).

J. Rohayem, M. Bergmann, J. Gebhardt, E. Gould, P. Tucker, A. Mattevi, T. Unge, R. Hilgenfeld & J. Neyts:
Antiviral strategies to control calicivirus infections.
Antiviral Res. 87, 162-178 (2010).

E. Wexselblatt, J. Katzhendler, R. Saleem-Batcha, G. Hansen, R. Hilgenfeld, G. Glaser & R. Vidavski:
ppGpp analogues inhibit synthetase activity of Rel proteins from Gram-negative and Gram-positive bacteria.
Bioorg. Med. Chem. 18, 4485-4497 (2010).

A. Rennenberg, C. Lehmann, A. Heitmann, T. Witt, G. Hansen, K. Nagarajan, C. Deschermeier, V. Turk, R. Hilgenfeld & V.T. Heussler:
Exoerythrocytic Plasmodium parasites secrete a cysteine protease inhibitor involved in sporozoite invasion and capable of blocking cell death of host hepatocytes.
PLoS Pathog. 6, e1000825 (2010).

H. Steuber & R. Hilgenfeld:
Recent Advances in Targeting Viral Proteases for the Discovery of Novel Antivirals.
Curr. Top Med. Chem. 23, 323-345 (2010).

S. Chen, F. Jonas, C. Shen & R. Hilgenfeld:
Liberation of SARS-CoV main protease from the viral polyprotein: N-terminal autocleavage does not depend on the mature dimerization mode.
Protein & Cell 1, 59-74 (2010).

N. Petermann, G. Hansen, C.L. Schmidt & R. Hilgenfeld:
Structure of the GTPase and GDI domains of FeoB, the ferrous iron transporter of Legionella pneumophila.
FEBS Lett. 584, 733-738 (2010).

M. Bollati, K. Alvarez, R. Assenberg, C. Baronti, B. Canard, S. Cook, B. Coutard, E. Decroly, X. De Lambellerie, E.A. Gould, G. Grard, J.M. Grimes, R. Hilgenfeld, A.M. Jansson, H. Malet, E.J. Mancini, E. Mastrangelo, A. Mattevi, M. Milani, G. Moureau, J. Neyts, R.J. Owens, J. Ren, B. Selisko, S. Speroni, H. Steuber, D.I. Stuart, T. Unge & M. Bolognesi:
Structure and Functionality in Flavivirus Ns-Proteins: Perspectives for Drug Design.
Antiviral Res. 87, 125-148 (2010).

Publications 2009

J.A. Wojdyla, I. Manolaridis, E.J. Snijder, A.E. Gorbalenya, B. Coutard, Y. Piotrowski, R. Hilgenfeld & P.A. Tucker:
Structure of the X (ADRP) domain of nsp3 from feline coronavirus.
Acta Cryst. D65, 1292-1300 (2009).

E. Liebau, K.F. Dawood, R. Fabrini, L. Fisher-Riepe, M. Perbandt, L. Stella, J.Z. Pedersen, A. Bocedi, P. Petrarca, G. Federici & G. Ricci:
Tetramerization and cooperativity in plasmodium falciparum glutathione transferase are mediated by the atypic loop 113-119.
J. Biol. Chem. 284, 22133-22139 (2009).

X. Tian, G. Lu, F. Gao, H. Peng, Y. Feng, G. Ma, M. Bartlam, K. Tian, J. Yan, R. Hilgenfeld & G.F. Gao:
Structure and cleavage-specificity of the chymotrypsin-like serine protease (3CLSP/nsp4) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV).
J. Mol. Biol. 392, 977-993 (2009).

H. Li, J. Huang, L. Chen, X. Liu, T. Chen, J. Zhu, W. Lu, X. Shen, J. Li, R. Hilgenfeld & H. Jiang:
Identification of Novel Falcipain-2 Inhibitors as Potential Antimalarial Agents through Structure-Based Virtual Screening.
J. Med. Chem. 52, 4936-4940 (2009).

J. Wolfova, I.K. Smatanova, J. Brynda, J.R. Mesters, M. Lapkouski, M. Kuty, A. Natalello, N. Chatterjee, S.-Y. Chern, E. Ebbel, A. Ricci, R. Grandori, R. Ettrich, & J. Carey:
Structural organization of WrbA in apo- and holo-protein crystals.
BBA-Proteins Proteom. 9, 1288-1298 (2009).

U. Mamat, H. Schmidt, E. Munoz, B. Lindner, K. Fukase, A. Hanuszkiewicz, J. Wu, T.C. Meredith, R.W. Woodard, R. Hilgenfeld, J.R. Mesters & O. Holst:
WaaA of the hyperthermophilic bacterium aquifex aeolicus is a monofunctional 3-deoxy-D-manno-oct-2-ulosonic acid transferase involved in lipopolysaccharide biosynthesis.
J. Biol. Chem. 284, 22248-22262, (2009).

P. Asanithi, E. Saridakis, L. Govada, I. Jurewicz, E.W. Brunner, R. Ponnusamy, J.A.S. Cleaver, A.B. Dalton, N.E. Chayen & R.P. Sear:
Carbon-Nanotube-Based Materials for Protein Crystallization.
ACS Appl. Mater. Interfaces. 6,1203–1210 (2009).

J. Tan, C. Vonrhein, O.S. Smart, G. Bricogne, M. Bollati, Y. Kusov, G. Hansen, J.R. Mesters, C.L. Schmidt & R. Hilgenfeld:
The SARS-unique domain (SUD) of SARS coronavirus contains two macrodomains that bind G-quadruplexes.
PLoS Pathog. 5, e1000428 (2009). (movie at www.scivee.tv)

A. Eichert, A. Schreiber, J.P. Fürste, M. Perbandt, C. Betzel, V.A. Erdmann & C. Förster:
Escherichia coli tRNAArg acceptor-stem isoacceptors: comparative crystallization and preliminary X-ray diffraction analysis.
Acta Cryst. F65, 98-101 (2009).

A. Eichert, M. Perbandt, A. Schreiber, J.P. Fürste, C. Betzel, V.A. Erdmann & C. Förster:
X-ray diffraction analysis of a human tRNA(Gly) acceptor-stem microhelix isoacceptor at 1.18 A resolution.
Acta Cryst. F65, 59-62 (2009).

Y. Piotrowski, G. Hansen, A.L. Boomaars-van der Zanden, E.J. Snijder, A.E. Gorbalenya & R. Hilgenfeld:
Crystal structures of the X-domains of a Group-1 and a Group-3 coronavirus reveal that ADP-ribose-binding may not be a conserved property.
Prot. Sci 18, 6-16 (2009).

Publications 2008

E. Liebau, J. Höppner, M. Mühlmeister, C. Burmeister, K. Lüersen, M. Perbandt, C. Schmetz, D. Büttner & N. Brattig:
The secretory omega-class glutathione transferase OvGST3 from the human pathogenic parasite Onchocerca volvulus.
FEBS J. 275, 3438-3453 (2008).

M. Perbandt, J. Höppner, C. Burmeister, K. Lüersen, C. Betzel & E. Liebau:
Structure of the extracellular glutathione S-transferase OvGST1 from the human pathogenic parasite Onchocerca volvulus.
J. Mol. Biol. 377, 501-511 (2008).

R. Ponnusamy, R. Moll, T. Weimar, J.R. Mesters & R. Hilgenfeld:
Variable oligomerization modes in coronavirus non-structural protein 9.
J. Mol. Biol. 383, 1081-1096 (2008).

I. Robel, J. Gebhardt, J.R. Mesters, A. Gorbalenya, B. Coutard, B. Canard, R. Hilgenfeld & J. Rohayem:
Functional characterization of the cleavage specificity of the Sapovirus chymotrypsin-like protease.
J. Virol. 82, 8085-8093 (2008).

R. Hilgenfeld, J. Tan, S. Chen, X. Shen & H. Jiang:
Structural proteomics of emerging viruses: The examples of SARS-CoV and other coronaviruses.
In Structural Proteomics (J. Sussman & I. Silman, eds.), 361-433 (2008).

K.H.G. Verschueren, K. Pumpor, S. Anemüller, S. Chen, J.R. Mesters & R. Hilgenfeld:
A Structural View of the Inactivation of the SARS-coronavirus main proteinase by benzotriazole esters.
Chem. Biol. 15, 597-606 (2008).

T. Klupsch, A. Walter, P. Mühlig & R. Hilgenfeld:
Combined kinetic osmometry and pyrometric microcalorimetry: Direct measurement of the protein-precipitant (salt) interaction.
Coll. Surf. A Physicochem. Eng. Aspects 318, 9-23 (2008).

T. Klupsch, A. Walter, P. Mühlig & R. Hilgenfeld:
Combined kinetic osmometry and pyrometric microcalorimetry on protein solutions: Setup and data evaluation.
Coll. Surf. A Physicochem. Eng. Aspects 318, 24-44 (2008).

M.F. Schmidt, A. Isidro-Llobet, M. Lisurek, A. El-Dahshan, J. Tan, R. Hilgenfeld & J. Rademann:
Sensitized Detection of Inhibitory Fragments and Iterative Development of Non-Peptidic Protease Inhibitors by Dynamic Ligation Screening.
Angew. Chem. Int. Ed. Engl. 47, 3275-3278 (2008).

Y. Piotrowski, R. Ponnusamy, S. Glaser, A. Daabach, R. Moll & R. Hilgenfeld:
Production of coronavirus nonstructural proteins in soluble form for crystallization.
In Methods in Molecular Biology 454, SARS- and other coronaviruses (D. Cavenagh, ed.),
Humana Press, Totowa, NJ (2008).

B. Coutard, A.E. Gorbalenya, E.J. Snijder, A.M. Leontovich, A. Poupon, X. De Lamballerie, R. Charrel, E.A. Gould, S. Gunther, H. Norder, B. Klempa, H. Bourhy, J. Rohayem, E. L'Hermite, P. Nordlund, D.I. Stuart, R.J. Owens, J.M. Grimes, P.A. Tucker, M. Bolognesi, A. Mattevi, M. Coll, T.A. Jones, J. Aqvist, T. Unge, R. Hilgenfeld, G. Bricogne, J. Neyts, P. La Colla, G. Puerstinger, J.P. Gonzalez, E. Leroy, C. Cambillau, J.L. Romette & B. Canard:
The VIZIER project: Preparedness against pathogenic RNA viruses.
Antiviral Res. 78, 37-46 (2008).

Publications 2007

J. Rupp, J. Gieffers, M. Klinger, G. van Zandbergen, R. Wrase, M. Maass, W. Solbach, J. Deiwick & T. Hellwig-Burgel:
Chlamydia pneumoniae directly interferes with HIF-1alpha stabilization in human host cells.
Cell Microbiol. 9, 2181-2191 (2007).

J. Tan, Y. Kusov, D. Mutschall, S. Tech, K. Nagarajan, R. Hilgenfeld & C.L. Schmidt:
The "SARS-unique domain" (SUD) of SARS coronavirus is an oligo(G)-binding protein.
Biochem. Biophys. Res. Commun. 364, 877-882 (2007).

J.R. Mesters & R. Hilgenfeld:
Protein glycosylation, sweet to crystal growth?
Cryst. Growth Des. 7, 2251-2253 (2007). Free PDF-download (registering is required).

J. Wolfova, J.R. Mesters, J. Brynda, R. Grandori, A. Natalello, J. Carey & I.K. Smatanova:
Crystallization and preliminary diffraction analysis of E. coli WrbA in complex with its cofactor flavin mononucleotide.
Acta Cryst. F63, 571-575 (2007).

D. Hubmacher, B.F. Matzanke & S. Anemüller:
Iron-uptake in the Euryarchaeon Halobacterium salinarum.
BioMetals 20, 539-547 (2007).

J.R. Mesters, K. Henning & R. Hilgenfeld:
Human glutamate carboxypeptidase II inhibition: structures of GCPII in complex with two potent inhibitors, quisqualate and 2-PMPA.
Acta Cryst. D63, 508-513 (2007).

T. Hogg & R. Hilgenfeld:
Protein Crystallography in Drug Discovery.
In: Comprehensive Medicinal Chemistry II (J.B. Taylor and D.J. Triggle, eds.)
Vol. 3 (ed. H. Kubinyi), pp. 875–900. Elsevier, Amsterdam (2007).

Publications 2006

J. Deiwick, S.P. Salcedo, E. Boucrot, S.M. Gilliland, T. Henry, N. Petermann, S.R. Waterman, J.P. Gorvel, D.W. Holden & S. Meresse:
The translocated Salmonella effector proteins SseF and SseG interact and are required to establish an intracellular replication niche.
Infect. Immun. 74, 6965-6972 (2006).

J.R. Mesters:
Practical Protein Crystallization.
In: Principles of Protein X-Ray Crystallography (Third Edition) by Jan Drenth.
Springer Science+Business Media LLC, New York, Chapter 16, pp. 297-304 (2006).

J.R. Mesters, J. Tan & R. Hilgenfeld:
Viral enzymes.
Curr. Opin. Struct. Biol. 16, 776-786 (2006).

G. Kaulmann, G.J. Palm, K. Schilling, R. Hilgenfeld, B. Wiederanders:
The crystal structure of a Cys25 -> Ala mutant of human procathepsin S elucidates enzyme-prosequence interactions.
Protein Sci. 15, 2619-2629 (2006).

R. Hilgenfeld, K. Anand, J.R. Mesters, Z. Rao, X. Shen, H. Jiang, J. Tan, K.H. Verschueren:
Structure and dynamics of SARS coronavirus main proteinase, (Mpro).
Adv. Exp. Med. Biol. 581, 585-591 (2006).

Y. Piotrowski, L. van der Hoek, K. Pyrc, B. Berkhout, R. Moll, R. Hilgenfeld:
Nonstructural proteins of human coronavirus NL63.
Adv. Exp. Med. Biol. 581, 97-100 (2006).

R. Ponnusamy, J.R. Mesters, J. Ziebuhr, R. Moll, R. Hilgenfeld:
Non structural proteins 8 and 9 of human coronavirus 229E.
Adv. Exp. Med. Biol. 581, 49-54 (2006).

N. Matthes, J.R. Mesters, B. Coutard, B. Canard, E. J. Snijder, R. Moll & R. Hilgenfeld:
The non-structural protein Nsp10 of Mouse Hepatitis Virus binds zinc ions and nucleic acids.
FEBS Lett. 580, 4143-4149 (2006).

J. Ondrácek & J.R. Mesters:
Ensemble of crystallographic models enabled description of novel bromate-oxoanion species, trapped within a protein crystal.
Acta Cryst. D62, 996-1001 (2006).

T. Hogg, K. Nagarajan, S. Herzberg, L. Chen, X. Shen, H. Jiang, M. Wecke, C.J. Blohmke, R. Hilgenfeld & C.L. Schmidt:
Structural and functional characterization of falcipain-2, a hemoglobinase from the malarial parasite plasmodium falciparum.
J. Biol. Chem. 281, 25425-25437 (2006).

S.I. Al-Gharabli, S.T. Shah, S. Weik, M.F. Schmidt, J.R. Mesters, D. Kuhn, G. Klebe, R. Hilgenfeld & J. Rademann:
An efficient method for the synthesis of peptide aldehyde libraries employed in the discovery of reversible SARS coronavirus main protease (SARS-CoV M(pro)) inhibitors.
ChemBioChem 7, 1048-1055 (2006).

R. Hilgenfeld & K. Pumpor:
Sometimes intermediates do the job!
Chem. Biol. 13, 235-236 (2006) (commentary).

J.R. Mesters, C. Barinka, W. Li, T. Tsukamoto, P. Majer, B.S. Slusher, J. Konvalinka & R. Hilgenfeld:
Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer.
EMBO J. 25, 1375-1384 (2006).

Publications 2005

D. Schneider, C.L. Schmidt:
Multiple rieske proteins in prokaryotes: Where and why?
Biochim. Biophys. Acta. 1710, 1-12 (2005).

H. Bönisch, C.L. Schmidt, P. Bianco, R. Ladenstein:
Ultrahigh-resolution study on Pyrococcus abyssi rubredoxin. I. 0.69 A X-ray structure of mutant W4L/R5S.
Acta Cryst. D61, 990-1004 (2005).

S. Reindel, C.L. Schmidt, S. Anemüller & B.F. Matzanke:
Expression and regulation pattern of ferritin-like DpsA in the archaeon Halobacterium salinarum.
Biometals 18, 387-397 (2005).

J. Tan, K.H.G. Verschueren, K. Anand, J. Shen, M. Yang, Y. Xu, Z. Rao, J. Bigalke, B. Heisen, J.R. Mesters, K. Chen, X. Shen, H. Jiang & R. Hilgenfeld:
pH-dependent conformational flexibility of the SARS-CoV main proteinase (Mpro) dimer: Molecular dynamics simulations and multiple X-ray structure analyses.
J. Mol. Biol. 354, 25-40 (2005).

H. Yang, W. Xie, X. Xue, K. Yang, J. Ma, W. Liang, Q. Zhao, Z. Zhou, D. Pei, J. Ziebuhr, R. Hilgenfeld, K.Y. Yuen, L. Wong, G. Gao, S. Chen, Z. Chen, D. Ma, M. Bartlam & Z. Rao:
Design of wide-spectrum inhibitors targeting coronavirus main proteases.
PLoS Biol. 3, 1742-1752 (2005).

D. Chakravortty, M. Rohde, L. Jäger, J. Deiwick, & M. Hensel:
Formation of a novel surface structure encoded by Salmonella Pathogenicity Island 2.
EMBO J. 24, 2043-2052 (2005).

K. Anand, H. Yang, M. Bartlam, Z. Rao & R. Hilgenfeld:
Coronavirus main proteinase: target for antiviral drug therapy.
In: Coronaviruses with special emphasis on first insights concerning SARS (A. Schmidt, M.H. Wolf & O. Weber, eds). Birkhäuser, Basel, pp. 173-199 (2005).

D.A. Groneberg, R. Hilgenfeld, P. Zabel:
Molecular mechanisms of severe acute respiratory syndrome (SARS).
Respir. Res. 6, 8-23 (2005).

Publications 2004

C. Luo, H. Luo, S. Zheng, C. Gui, L. Yue, C. Yu, T. Sun, P. He, J. Chen, J. Shen, X. Luo, Y. Li, H. Liu, D. Bai, J. Shen, Y. Yang, F. Li, J. Zuo, R. Hilgenfeld, G. Pei, K. Chen, X. Shen & H. Jiang:
Nucleocapsid protein of SARS coronavirus tightly binds to human cyclophilin A.
Biochem. Biophys. Res. Commun. 321, 557-565 (2004).

A. Hillisch, L.F. Pineda & R. Hilgenfeld:
Utility of homology models in the drug discovery process.
Drug Discov. Today 9, 659-669 (2004).

C. Gelhaus, R. Vicik, R. Hilgenfeld, C.L. Schmidt, M. Leippe & T. Schirmeister:
Synthesis and antiplasmodial activity of a cysteine protease-inhibiting biotinylated aziridine-2,3-dicarboxylate.
Biol. Chem. 385, 435-438 (2004).

T. Hogg, U. Mechold, H. Malke, M. Cashel, & R. Hilgenfeld:
Conformational antagonism between opposing active sites in a bifunctional RelA/SpoT homolog modulates (p)ppGpp metabolism during the stringent response.
Cell 117, 57-68 (2004).

C.L. Schmidt:
Rieske iron–sulfur proteins from extremophilic organisms.
J. Bioenerg. Biomembr. 36, 107-113 (2004).

R.G. Moll & G. Schäfer:
Novel functional aspects of the membrane-bound exo-pyrophosphatase of the hyperthermophilic Archaeon Sulfolobus are provided by analysis of its gene and the adjacent gene cluster.
J. Bioenerg. Biomembr. 36, 143-150 (2004).

R.G. Moll:
The archaeal signal recognition particle: steps towards membrane binding.
J. Bioenerg. Biomembr. 36, 47-53 (2004).

B.F. Matzanke, S. Anemüller, V. Schünemann, A.X. Trautwein & K. Hantke:
FhuF - part of a siderophore - reductase system.
Biochemistry 43, 1386-1392 (2004).

Publications 2003

T. Klupsch, P. Mühlig & R. Hilgenfeld:
The distribution of a macromolecular solute within an evaporating drop: an exact analytical solution.
Coll. Surf. A: Physicochem. Eng. Aspects 231, 85-102 (2003).

J.H. Helbig, B. König, H. Knospe, B. Bubert, C. Yu, C.P. Lück, A. Riboldi-Tunnicliffe, R. Hilgenfeld, E. Jacobs, J. Hacker & G. Fischer:
The PPIase active site of Legionella pneumophila Mip protein is involved in the infection of eukaryotic host cells.
Biol. Chem. 384, 125-137 (2003).

P. Mühlig, T. Klupsch, U. Kaulmann & R. Hilgenfeld:
Non-Invasive in situ Observation of the Crystallization Kinetics of Biological Macromolecules by Confocal Laser Scanning Microscopy.
J. Struct. Biol. 142 , 47-55 (2003).

H. Giersiefen, R. Hilgenfeld & A. Hillisch:
Modern methods of drug discovery: an introduction.
EXS 93 , 1-18 (2003).

A. Hillisch & R. Hilgenfeld:
The role of protein 3D-structures in the drug discovery process.
EXS 93 , 157-181 (2003).

K. Anand, J. Ziebuhr, P. Wadhwani, J.R. Mesters & R. Hilgenfeld:
Coronavirus Main Proteinase (3CLpro) Structure: Basis for Design of Anti-SARS Drugs.
Science 300, 1763-1767 (2003).

G. Kaulmann, G.J. Palm, K. Schilling, R. Hilgenfeld & B. Wiederanders:
An unfolding/refolding step helps in the crystallization of a poorly soluble protein.
Acta Cryst. D59, 1243-1245 (2003).

R. Kohler, J. Fanghanel, B. Konig, E. Luneberg, M. Frosch, J.U. Rahfeld, R. Hilgenfeld, G. Fischer, J. Hacker, M. Steinert:
Biochemical and functional analyses of the Mip protein: influence of the N-terminal half and of peptidylprolyl isomerase activity on the virulence of Legionella pneumophila.
Infect. Immun. 71, 4389-4397 (2003).

H. Yang, M. Yang, Y. Ding, Y. Liu, Z. Lou, Z. Zhou, L. Sun, L. Mo, S. Ye, H. Pang, G.F. Gao, K. Anand, M. Bartlam, R. Hilgenfeld & Z. Rao:
The crystal structures of severe acute respiratory syndrome virus main protease and its complex with an inhibitor.
Proc. Natl. Acad. Sci. USA 100, 13190-13195 (2003).

T. Hettmann, S. Anemüller, H. Borcherding, L. Mathé, P. Steinrücke & S. Dieckmann:
P. stuzeri soluble Nitrate Reductase alpha-beta subunit is a soluble enzyme with a similar electronic structure at the active site as the inner membrane bound alpha-beta-gamma holoenzyme.
FEBS Lett. 534, 143-150 (2003).

D. Hubmacher, B.F. Matzanke & S. Anemüller:
Effects of iron limitation on the respiratory chain and the membrane cytochrome pattern of the Euryarchaeon Halobacterium salinarum.
Biol. Chem. 384, 1565-1573 (2003).

R.G. Moll:
Protein-protein, protein-RNA and protein-lipid interactions of signal-recognition particle components in the hyperthermoacidophilic archaeon Acidianus ambivalens.
Biochem. J. 374, 247-254 (2003).

A. Hiller, T. Henninger, G. Schäfer & C.L. Schmidt:
New genes encoding subunits of a cytochrome bc1-analogous complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius.
J. Bioenerg. Biomembr. 35, 121-131 (2003).

D. Schneider, C.L. Schmidt & A. Seidler:
Expression of membrane-bound iron-sulfur proteins.
Methods Mol. Biol. 228, 37-47 (2003).

H. Gruegelsiepe, D.K. Willkomm, O. Goudinakis & R.K. Hartmann:
Antisense inhibition of Escherichia coli RNase P RNA: mechanistic aspects.
ChemBioChem. 4, 1049-1056 (2003).

D.K. Willkomm, H. Gruegelsiepe, O. Goudinakis, R. Kretschmer-Kazemi Far, R. Bald, V.A. Erdmann & R.K. Hartmann:
Evaluation of bacterial RNase P RNA as a drug target.
ChemBioChem 4,1041-1048 (2003).

T. Persson, S. Cuzic & R.K. Hartmann:
Catalysis by RNase P RNA: unique features and unprecedented active site plasticity.
J. Biol. Chem. 278, 43394-43401 (2003).

R. Feltens, M. Gossringer, D.K. Willkomm, H. Urlaub & R.K. Hartmann:
An unusual mechanism of bacterial gene expression revealed for the RNase P protein of Thermus strains.
Proc. Natl. Acad. Sci. USA 100, 5724-5729 (2003).

C. Kruse, D. K. Willkomm, J. Gebken, A. Schuh, H. Stossberg, T. Vollbrandt & P.K. Müller:
The multi-KH protein vigilin associates with free and membrane-bound ribisomes.
Cell Mol. Life Sci. 60, 2219-2227 (2003).

E. Hartmann & R.K. Hartmann:
The enigma of ribonuclease P evolution.
Trends Genet. 19, 561-569 (2003).

Publications 2002

K. Anand, G.J. Palm, J.R. Mesters, S.G. Siddell, J. Ziebuhr & R. Hilgenfeld:
Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain.
EMBO J. 21, 3213-3224 (2002).

T. Hogg, J.R. Mesters & R. Hilgenfeld:
Inhibitory Mechanisms of Antibiotics Targeting Elongation Factor Tu.
Curr. Prot. Pept. Sci. 3, 121-131 (2002).

J. Weber, J.R. Mesters, M. Lepsik, J. Prejdova, M. Svec, J. Sponarova, P. Mlcochova, K. Skalicka, K. Strisovsky, T. Uhlikova, M. Soucek, L. Machala, M. Stankova, J. Vondrasek, T. Klimkait, H.-G. Kraeusslich, R. Hilgenfeld & J. Konvalinka:
Unusual Binding Mode of an HIV-1 Protease Inhibitor Explains its Potency against Multi-drug-resistant Virus Strains.
J. Mol. Biol. 324, 739-754 (2002).

E.B. Küttner, R. Hilgenfeld & M.S. Weiss:
Purification, characterization, and crystallization of alliinase from garlic.
Arch. Biochem. Biophys. 402, 192-200 (2002).

E.B. Küttner, R. Hilgenfeld & M.S. Weiss:
The active principle of garlic at atomic resolution.
J. Biol. Chem. 277, 46402-46407 (2002).

T. Sicker & R. Hilgenfeld:
Blutgerinnungsfaktor XIII: Aktivierung, Substrate und Struktur einer Transglutaminase.
Hämostaseologie 1/2002, 8-13 (2002).

K. Anand, D.Pal & R. Hilgenfeld:
An overview on 2-methyl-2,4-pentanediol in crystallization and in crystals of biological macromolecules.
Acta Cryst. D58, 1722-1728 (2002).

N.E. Chayen & R. Hilgenfeld:
Crystallization comes of age.
Trends Biotechn. 20, 320-321 (2002).

T. Hogg. I. Kuta Smatanova, K. Bezouska, N. Ulbrich & R. Hilgenfeld:
Sugar-mediated lattice contacts in crystals of a plant glycoprotein.
Acta Cryst. D58, 1734-1739 (2002).

M. Riess-Kautt & R. Hilgenfeld (eds.):
Proceedings of the 9th International Conference on the Crystallization of Biological Macromolecules.
Acta Cryst. D58, 1511-1764 (2002).

G. Webster & R. Hilgenfeld:
Perspectives on Single Molecule Diffraction Using the X-Ray Free Electron Laser.
Single Mol. 3, 61-66 (2002).

A. Hillisch & R. Hilgenfeld (eds.):
Modern Methods in Drug Discovery.
Birkhäuser-Verlag. Basel, ISBN 3-7643-6081-X (2002).

Publications 2001

A. Riboldi-Tunnicliffe, B. König, S. Jessen, M.S. Weiss, J. Rahfeld, J. Hacker, G. Fischer & R. Hilgenfeld:
Crystal structure of Mip, a prolylisomerase from Legionella pneumophila.
Nat. Struct. Biol. 8, 779-783 (2001).

L. Vogeley, G.J. Palm, J.R. Mesters & R. Hilgenfeld:
Conformational change of elongation factor Tu induced by antibiotic binding: Crystal structure of the complex between EF-Tu:GDP and aurodox.
J. Biol. Chem. 276, 17149-17155 (2001).

J.R. Mesters, T. Hogg & R. Hilgenfeld:
G proteins.
In: Encyclopedia of Life Sciences. Macmillan (Nature), London, 2001 (www.els.net).

A. Ambrus, I. Banyai, M.S. Weiss, R. Hilgenfeld, Z. Keresztessy, L. Muszbek & L. Fesus:
Calcium binding to transglutaminases: A 43Ca NMR study combined with surface polarity analysis.
J. Biomol. Struct. Dyn. 19, 1-15 (2001).

M.S. Weiss, M. Brandl, J. Sühnel, D. Pal & R. Hilgenfeld:
More hydrogen bonds for the (structural) biologist.
Trends Biochem. Sci. 26, 521-523 (2001).

M. Brandl. M.S. Weiss, J. Sühnel & R. Hilgenfeld:
C-H...π interactions in proteins.
J. Mol. Biol. 307, 357-377 (2001).

A. Rau, T. Hogg, R. Marquardt & R. Hilgenfeld:
A new lysozyme fold: Structure of the muramidase from Streptomyces coelicolor at 1.65 Å resolution.
J. Biol. Chem. 276, 31994-31999 (2001).

M.S. Weiss, T. Sicker & R. Hilgenfeld:
Soft X-rays, high redundancy and proper scaling: A new procedure for automated protein structure determination via SAS.
Structure 9, 771-777 (2001).

M.S. Weiss, T. Sicker, K. Dijnovic-Carugo & R. Hilgenfeld:
On the routine use of soft X-rays in macromolecular crystallography.
Acta Cryst. D57, 689-695 (2001).

G. Webster & R. Hilgenfeld:
An evolutionary computational approach to solving the phase problem in macromolecular crystallography.
Acta Cryst. A57, 351-358 (2001).

P. Mühlig, T. Klupsch, U. Schell & R. Hilgenfeld:
Investigation of the early stages of insulin crystallization by Confocal Laser Scanning Microscopy (CLSM).
J. Cryst. Growth 232, 93-101 (2001).